Talk:CH391L/S14/Ella's First assignment

Worksheet: This should take you about 20 to 30 minutes to complete, including reading the wiki. Use whatever space you need. [ask for the word file, if you do not have it] Overall Format and structure:

Introduction and background material: Would like to see more information about the other types of restriction enzymes. Potentially add a diagram about how the type II enzymes cleave palindromic sequences. A link to some information about type 3 and 4 https://www.neb.com/products/restriction-endonucleases/restriction-endonucleases/types-of-restriction-endonucleases It seems incomplete to mention that there are four types and not elaborate a little bit on what the other ones are, and why or why not they are useful.

Methods and main body/concepts: Would like to see more detail about the NEB cutter, i.e. what does it do? It takes non-overlapping reading frames and identifies regions in E. Coli genetic code where type II restriction enzymes will cut. (http://tools.neb.com/NEBcutter2/index.php) Another thing to elaborate on: The information on BioBrick/BglBrick assembly standards is informative, however the use seems to be unclear if there is an immediate connection to the previous information. Either there needs to be seguing to the biobricks or there is a need for an introductory paragraph for the Biobrick. Coming from knowing nothing about bicbricks, I could not understand the point of the “standards.” Must elaborate on why standards are necessary. It also wouldn’t hurt to have a brief description about what biobricks basically are. Why is it important we have standards? The goal is to standardize a system for other scientists to use and information can be passed on without confusion. Explain what RFCs are. There is a small paragraph about importance.

Relation to iGEM and future directions: There is no section that introduce any relation or future directions.

Figures, Figure legends, and citations: Dennis mentioned that the citations in many of the older wiki pages are not done well. Now, it is known that underneath there needs to be a small description of the article rather than the article name itself. In first figure the terminology should also mention "palindromic sequences," as it is a more accurate term for "inverted-repeat symmetry."

WHEN FINISHED, save the file and e-mail it to dennis.mishler@gmail.com. This is due BEFORE 9:00 AM Friday. Instructor Mishler will then add the revisions to the wiki page and e-mail the worksheet to the author, who will revise the wiki in response to the critiques on this worksheet before the next class.