Talk:CH391L/S14/BioBricks

Missing components

 * --Dst465 (talk) 14:02, 27 January 2014 (CST) "Ligases:" There is an in depth discussion and coverage of restriction enzymes, yet there is no actually discussion on the importance of ligases, which are required for assembly.
 * --Ew6977 (talk) 15:26, 30 January 2014 (CST) Added a subsection on the role of ligase in a natural bacterial cell and also how it is utilized in synthetic bio


 * --Dennis Mishler (talk) 13:04, 28 January 2014 (CST) In the section covering prefix and suffix, you mention "contains vector insert restriction sites" this is a little confusing. Could you perhaps reword this to be more precise in your meaning?


 * --Dennis Mishler (talk) 13:04, 28 January 2014 (CST)Also, in the paragraph immediately above, there is a typo "PtsI" instead of "PstI".  You may want to check for other grammatical/spelling errors throughout the document.
 * --Ew6977 (talk) 14:24, 30 January 2014 (CST)Fixed that typo- it turns out I have been saying it wrong this whole time (I have been saying "PtsI," so surely that is why I spelled it wrong


 * --Dennis Mishler (talk) 13:17, 28 January 2014 (CST) Ella, there is no section on the topic's relation to iGEM/future directions.
 * --Ew6977 (talk) 14:49, 30 January 2014 (CST) I added one, sorry when I originally did the paper I did not think that the biobrick standard and fixing DNA within plasmids had very much to go forward with. Upon talking with Mike I realized that there are a LOT of things we can improve upon! The more I learn, the more little connections I make, the better I can understand how we can take these things to the next steps.


 * --Aeg2338 (talk) 20:33, 30 January 2014 (CST) I don't know how important this is, but I would like to know a little more about the ideas people are working on to fix the issues associated with the current system (mentioned at the end of the future directions section). Maybe add an example of a potential new method? I'm not sure how much is out there about it, but it would help me understand the other potential methods of designing BioBricks being offered and discussed.
 * --Ew6977 (talk) 23:54, 30 January 2014 (CST) I actually had to email a current iGEM member to get the answer and was waiting for her reply to add it! I did add the information in the future directions section


 * --Ajv684 (talk) 21:41, 30 January 2014 (CST) There is a typo in section "The creation of a standard..." Second paragraph, first line reads "...joint..." and should read "...join...". In general, it is a pretty good job. I find personally a bit confusing how BioBricks and iGEM registry were treated since for someone with no previous knowledge of this, it might be really difficult to find out what the relationship between these two is. It was confusing for me at least. Also, improving clarity probably in communicating the complexity of this standardization technique by making a simpler figure with a more explanatory caption might help the reader understand better. Finally, I think there was a lot spent in explaining the restriction enzymes that I find an easier topic as opposed to biobricks that I am less familiar with. But these all are probably stylistic suggestions, in general I think the wiki was well done, good formatting, good structure and nice wording.
 * --Ew6977 (talk) 23:54, 30 January 2014 (CST) Fixed typo, and I will look into how to rephrase some of the connections, I myself struggled to decipher all of the information and I hope that at least I elucidated at least some.

Overall Format and structure: The overall content is very informative and the writing is professional. The format is good and reader should be able to understand the background and basic idea of how restriction endonucleases work.
 * --Dennis Mishler (talk) 08:54, 31 January 2014 (CST) CHEN's wiki critique:

However, the main content is somewhat limited to how to build a standard sequence of iGEM, and doesn’t say much about a more general background such as the history of iGEM, how it applies to current researches and some example of what can Biobrick actually do.

Introduction and background material: The introduction section does provide the necessary material for readers to understand what BioBrick/restriction endonucleases are and why it is important the biosynthetic community. On the other hand, I think the author can also discuss few examples of Biobrick, such as the most commonly used or the earlier developed BioBrick.

Methods and main body/concepts: The main body is mainly discussing how to build the prefix and suffix and the type of restriction endonucleases. Those are good but other topics such as why bacteria need those enzymes and how people actually use BioBricks in their research or real application will make the story more complete.

Relation to iGEM and future directions: This page is actually talking about a project of iGEM so I think it did a good job here. For future direction, the author can provide more information of how BioBrick applies in the cutting edge research or any novel finding of restriction endonucleases.

Figures, Figure legends, and citations: The figures are easy to understand and very helpful for the reader. They are placed right next to the related topic and easy to read. The citations are clear and helpful. A minor thing is the illustration of a Biobrick is too small to read and might need a more detailed legend.