DNA Assembly Methods(Microbes as Machines)

[[Media:Spring2015_DNA_Assembly_Methods_Pre-Discussion_Questions.pdf| Download Pre-Discussion Questions (PDF)]]


The only DNA in the cells is the designed synthetic DNA sequence, including “watermark” sequences and other designed gene deletions and polymorphisms, and mutations acquired during the building process Gibson2010.

= Required Reading/Viewing =

// Construction of synthetic Mycoplasma laboratorium genome.
 * 1) Gibson2010 pmid=20488990
 * 1) Venter2010 Venter press conference / TED Talk discussing this paper

= Assigned Papers =

// Oligonucleotide and gene synthesis methods // Isothermal DNA assembly (aka Gibson Cloning). Additional information on the AddGene website. // 1/2 In vivo DNA assembly using homologous recombination in yeast // 2/2 In vivo assembly of single-stranded oligonucleotides in yeast
 * 1) Hughes2011 pmid=21601682
 * 1) Gibson2009a pmid=19363495
 * 1) Kuijpers2013 pmid=24028550
 * 1) Gibson2009b pmid=19745056

= Additional Information =